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L-ascorbate biosynthesis VIII (engineered pathway)

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
2-dehydro-D-gluconate + acceptor <=> reduced_acceptor + 2,5-didehydro-D-gluconate 1.1.99.4 dehydrogluconate dehydrogenase

D-glucono-1,5-lactone + H2O <=> H+ + D-gluconate 3.1.1.17 gluconolactonase
3.1.1.35 dihydrocoumarin hydrolase


D-gluconate + acceptor <=> 2-dehydro-D-gluconate + reduced_acceptor 1.1.99.3 gluconate 2-dehydrogenase (acceptor)
1.1.99.6 D-lactate dehydrogenase (acceptor)


2-dehydro-D-gluconate + NADP+ <=> H+ + NADPH + 2,5-didehydro-D-gluconate 1.1.1.274 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
1.1.1.346 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)


2-keto-L-gulonate + NADP+ <=> 2,5-didehydro-D-gluconate + NADPH + H+ 1.1.1
1.1.1.274 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
1.1.1.346 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)



ubiquinone + D-glucose <=> ubiquinol + D-glucono-1,5-lactone 1.1.5.2 glucose 1-dehydrogenase (PQQ, quinone)
1.1.5.9 glucose 1-dehydrogenase (FAD, quinone)


ascorbate + H2O <=> 2-keto-L-gulonate not assigned

Visualization of the pathway